Primers for amplification of AIM-1

region	Primer name	sequence	Annealing temp (°C)
Exon 1	AIM-1 ex1 F AIM-1 ex1 R	TCCAGCACCACCCCCTCCCTCTCAT TGCAATGCCAGAAAGGTCCTCTTGA	59
Exon 2	AIM-1 ex2 F AIM-1 ex2 R	AAAAGCAAAATAAAAGCAAGGAAGA AGGCTTCTTTCCATTTCAGTTGTAA	55
Exon 3	AIM-1 ex3 F AIM-1 ex3 R	CTCTGCTGTCTTCAGGGAGTTTTCC ACCAACCCAAGGATGCTACCATTTG	56
Exon 4	AIM-1 ex4 F AIM-1 ex4 R	CACTTTGTGTGATGGCTGACTGACA ATTAAATACTGGCAGGCTCCGTAGT	56
Exon 5	AIM-1 ex5 F AIM-1 ex5 R	GATGTGATAGGATGCGACTTGGTCA GGGACTCATCCATCCAACACTTCAT	56
Exon 6	AIM-1 ex6 F AIM-1 ex6 R	CCACAGATAAGGGGATTCTTTTGTT ATGGTTGCAGTTGGTTGGGCATTTG	56
Exon 7	AIM-1 ex7 F AIM-1 ex7 R	TTCAGTTCAGTTCCACATCACCAAT GGGCGTCCAATGGGCAGACCCTAAG	53.5
Exon 3 RFLP	AIM-1 ex3 F AIM-1 ex3 RF-R	CTCTGCTGTCTTCAGGGAGTTTTCC CGCCGCCGCCAATGTTGCATCTTTACCTGTTCAGC	55

The temperature profile of PCR was at 94°C for 3 min followed by 35 cycles at 96°C for 15 sec, at X°C (each listed in this table) for 30 sec and at 72°C for 1 min.