Primer sequence and DHPLC conditions

Amplicon	Amplified region	Primer sequence (5’-3’)	size(bp)	Annealing Temp (°C)	Oven temp (°C)	% B buffer start/end
Int 4	32638-32889	CTGTGAGATGGAAGGAGGAATGTTA GAGAGATTGGAGGCTGTTGAGGTAC	252	59.4	58.3	53.1-62.1
Ex 5	32732-33016	GATGTGATAGGATGCGACTTGGTCA AATAAAGTGAGGAAAACACGGAGTT	285	57.0	58.1	54.3-63.3
Int 5A	32931-33320	CTCCACAGAGTTTCTCATCTACGAA GGGACTCATCCATCCAACACTTCAT	390	61.6	55.6	56.9-65.9
Int 5B	33225-33613	ATTACTTTGCTCTTGGAATCAGTGG AAAGACAATAGVAGAAATCCCTTTG	389	58.3	57.0	56.8-65.8
Int 5C	33589-33902	GCCAGCTTTTCTGAGCCTCTTAACC GAATTGGGGGTCAGTTATTCTTACA	314	64.0	58.0	56.6-65.6

The temperature profile of PCR was at 95°C for 2 min followed by 35 cycles at 95°C for 30 sec, at X°C (each listed in this table) for 30 sec and at 72°C for 40 sec.